20× SSC buffer

To prepare 1 L of 20× SSC buffer

Component CAS Molecular Weight Weight Concentration
Sodium Chloride 7647-14-5 58.44 g/mol 175.3 g 3 M
Sodium citrate 6132-04-3 294.10 g/mol 77.4 g 0.3 M
  • Dissolve chemical in 800 mL of ddH2O.
  • Adjust pH to 7.0 with HCl.
  • Adjust volume to 1 L.
  • Sterilize by autoclaving.